Hi Rhys -
Don't forget to try sulfur-SAD, especially the multi-crystal version
published recently:
http://journals.iucr.org/d/issues/2013/07/00/ba5189/index.html
This seems well suited to your situation.
- Matt
On 1/15/14 12:18 PM, RHYS GRINTER wrote:
Hello message board,
My group has some crystals of an interesting protein to take to the synchrotron
in a couple of weeks. We won't be able to prepare and crystallise a SelMet
derivative during that time period, but we have loads of crystals sitting
around. The diffraction isn't great, we see maybe 3.5 at home but might be
enough to get over the line.
It will be a very difficult MR target, so we were thinking of soaking so
crystals with heavy atomic compounds that we have lying around. I was wondering
if people had any suggestions of compounds that people have used successfully
for experimental phasing and maybe concentrations to use and soaking time.
Cheers,
Rhys
--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374
