And quick iodide soaks may be useful in the 200 mM range. See the sped-up video: http://www.youtube.com/watch?v=45Qc3jOPaKY
Quiz time: What wavelength would give iodide a similar signal to that of selenium? Can one get a better signal than selenium by choosing a different wavelength for data collection? and of course the webinar presented by Thomas Edwards of the SSGCID: http://www.rigaku.com/node/1369 ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of RHYS GRINTER [r.grinte...@research.gla.ac.uk] Sent: Wednesday, January 15, 2014 11:18 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Best compounds for heavy atom soaks Hello message board, My group has some crystals of an interesting protein to take to the synchrotron in a couple of weeks. We won't be able to prepare and crystallise a SelMet derivative during that time period, but we have loads of crystals sitting around. The diffraction isn't great, we see maybe 3.5 at home but might be enough to get over the line. It will be a very difficult MR target, so we were thinking of soaking so crystals with heavy atomic compounds that we have lying around. I was wondering if people had any suggestions of compounds that people have used successfully for experimental phasing and maybe concentrations to use and soaking time. Cheers, Rhys
