Hi Rhys, It's worth paying close attention to your crystallisation conditions as well - some heavy atom compounds will not be at all soluble in very alkaline (they'll form insoluble hydroxides) or phosphate/sulphate containing mother liquors.
A very low pH may reduce the binding efficiency of some heavy atom compounds as well - many heavy atom compounds rely on having deprotonated side chains to interact with - if you are at pH 5 or lower, everything save your Asps and Glus are likely to be protonated and you'll perhaps have better luck with Lanthanides (or Uranyl salts). Good luck! Dave ============================ David C. Briggs PhD http://about.me/david_briggs On 16 January 2014 09:53, RHYS GRINTER <r.grinte...@research.gla.ac.uk>wrote: > Hi All, > > A truly herculean response! Thanks everyone, I will process all of the > information and come up with a strategy. > > Rhys >
