On Thu, 2013-08-22 at 14:22 -0400, Bosch, Juergen wrote:
> But when it sits and crystallizes it is cleaner - unless some
> opportunistic fungal contamination helps you trim off those nasty
> loops that you would have omitted anyhow from your model.
Jurgen,
Good point and admittedly I never considered that. On the other hand,
when supernatant is injected in the column on FPLC, other components
that may harm the protein are either rapidly removed from the
environment or immobilized on the resin and can do no further damage.
Does this mean that the batch method exposes protein for longer time as
you incubate it with the resin?
Of course, we are splitting hairs (at least I am) - both methods work
just fine most of the time and one can find problematic examples with
both.
Cheers,
Ed.
--
Oh, suddenly throwing a giraffe into a volcano to make water is crazy?
Julian, King of Lemurs
