Yes, I think that's the one we use now. I believe it is the current branding of the Fluka imidazole that we always ordered. Definitely lower UV absorbance. We also prefer Roche high purity DTT for the same reason.
John Sent from my iPad On Aug 21, 2013, at 11:04 AM, Jan <jan.abendr...@gmail.com> wrote: > Hi Bernhard, > this one works for us: > Sigma BioUltra imidazole, >99.5% by GC, 56749 > > Cheers, > Jan > -- > Jan Abendroth > Emerald BioStructures > Seattle / Bainbridge Island WA, USA > home: Jan.Abendroth_at_gmail.com > work: JAbendroth_at_embios.com > http://www.emeraldbiostructures.com > > On Aug 21, 2013, at 7:33 AM, Bernhard Rupp <hofkristall...@gmail.com> wrote: > >> Hi Fellows, >> >> could someone please point me towards the source of a known high purity >> imidazole >> with low absorbance at 280 nm? I am facing the problem of detecting a low >> absorption protein >> in high imidazole background after IMAC gradient elution. In the UV spectra >> of the >> 2 imidazoles I checked there is some contaminant that absorbs at 280… >> >> Thx, BR >> >> ---------------------------------------------------------------------------------------- >> Bernhard Rupp >> Marie Curie Incoming International Fellow >> Innsbruck Medical University >> Schöpfstrasse 41 >> A 6020 Innsbruck – Austria >> +43 (676) 571-0536 >> bernhard.r...@i-med.ac.at >> ---------------------------------------------------------------------------------------- >> Dept. of Forensic Crystallography >> k.-k. Hofkristallamt >> Vista, CA 92084 >> 001 (925) 209-7429 >> b...@ruppweb.org >> b...@hofkristallamt.org >> http://www.ruppweb.org/ >> ----------------------------------------------------------------------- >
