But when it sits and crystallizes it is cleaner - unless some opportunistic
fungal contamination helps you trim off those nasty loops that you would have
omitted anyhow from your model.
Jürgen
P.S. I like kangaroo's in particular when served with red wine and medium rare
next to some garlic mashed potatoes
On Aug 22, 2013, at 2:17 PM, Ed Pozharski wrote:
On Thu, 2013-08-22 at 13:58 -0400, Bosch, Juergen wrote:
well if we hit the timer after lysis, say via cell disruptor then I
have my eluted protein in less than 1 hour, including 40 minutes batch
binding.
then proceeds to wait six weeks for crystals to appear... :)
Cheers,
Ed.
PS. There are many ways to skin a kangaroo and ultimately indeed all
things serve the Beam.
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
http://lupo.jhsph.edu
