Hi everybody,
I would just like to contribute that absorption at 280 results from
methylated imidazole, a contamination hard to get rid of. Imidazole
itself does not absorb at 280.
Bye,
Matthias
-----------------------------------------
Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Fax (+44) 1865 287547
Email matth...@strubi.ox.ac.uk
Website http://www.strubi.ox.ac.uk
-----------------------------------------
On 8/21/2013 7:43 PM, Bosch, Juergen wrote:
How about low pH elution or EDTA as alternative ?
Jürgen
......................
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://lupo.jhsph.edu
On Aug 21, 2013, at 14:40, "Prof. Dr. Arne Skerra" <ske...@tum.de
<mailto:ske...@tum.de>> wrote:
Hi Bernhard,
We know this problem since the very early days of the His-tag and
have been using high purity imidazole from Merck since then with
success, which essentially shows no absorption at 280 nm. If you like
I can enquire the current order number in my lab.
Cheers, ASk
Am 21.08.2013 um 16:33 schrieb Bernhard Rupp:
Hi Fellows,
could someone please point me towards the source of a known high
purity imidazole
with low absorbance at 280 nm? I am facing the problem of detecting
a low absorption protein
in high imidazole background after IMAC gradient elution. In the UV
spectra of the
2 imidazoles I checked there is some contaminant that absorbs at 280…
Thx, BR
----------------------------------------------------------------------------------------
Bernhard Rupp
Marie Curie Incoming International Fellow
Innsbruck Medical University
Schöpfstrasse 41
A 6020 Innsbruck – Austria
+43 (676) 571-0536
bernhard.r...@i-med.ac.at <mailto:bernhard.r...@i-med.ac.at>
----------------------------------------------------------------------------------------
Dept. of Forensic Crystallography
k.-k. Hofkristallamt
Vista, CA 92084
001 (925) 209-7429
b...@ruppweb.org <mailto:b...@ruppweb.org>
b...@hofkristallamt.org <mailto:b...@hofkristallamt.org>
http://www.ruppweb.org/
-----------------------------------------------------------------------
*
Prof. Dr. Arne Skerra*
Lehrstuhl f. Biologische Chemie | Technische Universitaet Muenchen
Emil-Erlenmeyer-Forum 5 | 85350 Freising-Weihenstephan | Germany
Phone: +49 (0)8161 71-4351 | Fax: -4352
http://www.wzw.tum.de/bc | eMail: ske...@tum.de <mailto:ske...@tum.de>
