Hello, I have a question about molecular replacement.
I use "Phaser" or "AutoMR" to generate models of my target protein. Input .mtz is from X-ray diffraction. Template is from a known structure. I also set up seq file using my target protein. The sequence identity between template and my target protein is quite high, over 90%. When I exam the ouputs, I found the sequence of the output .pdb is exactly same as the template. Is this normal for Molecular replacement? In order to have my target .pdb, I need to mutate the residues using coot? Thank you for advice Ros
