*CALL FOR PROPOSALS FOR ESRF BEAM TIME WITH ONLINE MICROSPEC*
Proposal Deadline **1st May 2012**
There will be beam time available at the ESRF for MX data collectionwith
a setup that allows online monitoring of UV/VIS absorbance
orfluorescence spectral changes of the crystal during the
X-raydiffraction experiment. Users who are interested in using this beam
time(including those who are members of BAG Groups) should use the
followingmechanism:_http://www.esrf.fr/UsersAndScience/UserGuide/Applying/ProposalGuidelines/MXnon-BAGproposal_and
*it must be clearly indicated in the title of the proposal form
that**the online monitoring of spectral changes is necessary for the
project*. A brief description of the device is given below however users
areencouraged to consult the web pages for detailed
information:_http://www.esrf.fr/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/Run_Your_Experiment/Microspectrophotometer_User_Guide_The
device is also described in: McGeehan, J., Ravelli, R.B., Murray,J.W.,
Owen, R.L., Cipriani, F., McSweeney, S., Weik, M. and Garman, E.F.(2009)
Colouring cryo-cooled crystals: online microspectrophotometry.
JSynchrotron Radiat., 16, 163-172.
As this is not a standard set-up, it might take a significant amount
oftime to train users, align the device, and analyze the data in order
toderive relevant data collection schemes. *We will therefore schedule
24**hours for each project*. The *deadline *for this specific
application is*Tuesday 1st**May 2012*.
It is strongly recommended to, beforehand, record an
absorption(fluorescence) spectrum of the crystal on a home
microspectrophotometersuch as the 4dx one, or at an off-line facility
such as the ESRFCryobench, and to provide it in the application form.
Such a spectrumwould greatly help to determine the feasibility of the
experiment. Foroptimal experimental conditions, crystals should be
frozen in minimalamounts of cryosolution, especially when the crystals
are small.Finally, please note that *the ESRF sample changer cannot be
operated at**the same time as the on-line microspec*.The use of specific
LASER is possible if the device is compliant withthe beam line safety
system (interlock on device power).
++++++++++++
Dates of beam-time: *13th June - 17th June 2012*
Storage Ring: Uniform (200mA)
Beamline: ID14-1
Energy: 13.27 keV (not tunable)
Specifications:
UV/VIS-range: 250-1100 nm
Light source: Mikropack DH-2000-BAL (Deuterium/Halogen)
Fluorescence/Actinic excitation wavelength: 405, 440, 473, 532, 561, 671 nm
ODmax for UV-vis absorbance spectra: 2-2.5
Monitoring light size: 0.03 (min) - 0.15mm(max)
Sampling freq (to disk): 10Hz or lower
--
Dr David FLOT
Beam-Line Operation Manager Tel : (+33) 4 76 88 17 63
Structural Biology Group Fax : (+33) 4 76 88 26 24
ESRF
B.P. 220, 6 rue Jules Horowitz e-mail : david.f...@esrf.fr
F-38043 GRENOBLE CEDEX http://www.esrf.eu
