On Mon, Oct 17, 2011 at 10:09 AM, Napoleão Valadares <n...@ifsc.usp.br>wrote:
> I have tried A LOT of Molecular Replacement using Phaser and Phenix > AutoMR. I'm using a 80% identity coiled coil helix as search model. The > programs give me solutions with "reasonable" maps, but it is never possible > to refine to achieve Rvalues below 0.40. Additionally, maps from different > solutions look reasonable, so I'm thinking these are all bias. > Did you try running an automated building program (phenix.autobuild or ARP/wARP)? If the solutions are real, the software should do a very good job, much better than refinement alone. Otherwise, I agree ARCIMBOLDO sounds like a good choice here. -Nat
