I think I know another protein that does this: gelatin! (Well, not the crystallization part...)
Jacob On Fri, Sep 16, 2011 at 2:41 PM, Phoebe Rice <pr...@uchicago.edu> wrote: > Gamma delta resolvase catalytic domain stock solutions used to make a nice > clear jelly at 4 degrees, but it was perfectly reversible by warming the > sample to room T. In fact, one mutant crystallized in the stock tube after a > few trips in and out of the fridge. The crystals didn't diffract very well > (so we never published that), but it was a fun way to grow them. > Phoebe > > ===================================== > Phoebe A. Rice > Dept. of Biochemistry & Molecular Biology > The University of Chicago > phone 773 834 1723 > http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 > http://www.rsc.org/shop/books/2008/9780854042722.asp > > > ---- Original message ---- >>Date: Tue, 30 Aug 2011 23:31:20 +0800 >>From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of aidong >><a...@xmu.edu.cn>) >>Subject: [ccp4bb] Protein preps become a jelly >>To: CCP4BB@JISCMAIL.AC.UK >> >>Dear Buddies, >> >>Sorry for bothering you with an off-ccp4 question. We recently are >>experiencing a very strange phenomena. A couple of protein preps with >>reasonably high concentration (10-20mg/ml) become a jelly after >>storages for overnight or a couple of days at 4C. All of them have >>been purified by gel filtration. Some of these proteins behave like >>this from very first preps but some of them had been very kind to us >>previously. We have googled extensively in CCP4BB and www but it >>appears this only happens to us. It would be highly appreciated that >>you could exchange their experiences or provide your suggestions. >> >>Aidong Han, Ph.D >> >>Department of Biomedical Sciences >>School of Life Sciences >>Xiamen University >>Xiamen, Fujian 361005 >>China >>Phone: 0592-218-8172 (O) >> 0592-218-8173 (L) >>Web: http://life.xmu.edu.cn/adhanlab/ > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: j-kell...@northwestern.edu *******************************************
