Dear Buddies,
Sorry for bothering you with an off-ccp4 question. We recently are
experiencing a very strange phenomena. A couple of protein preps with
reasonably high concentration (10-20mg/ml) become a jelly after
storages for overnight or a couple of days at 4C. All of them have
been purified by gel filtration. Some of these proteins behave like
this from very first preps but some of them had been very kind to us
previously. We have googled extensively in CCP4BB and www but it
appears this only happens to us. It would be highly appreciated that
you could exchange their experiences or provide your suggestions.
Aidong Han, Ph.D
Department of Biomedical Sciences
School of Life Sciences
Xiamen University
Xiamen, Fujian 361005
China
Phone: 0592-218-8172 (O)
0592-218-8173 (L)
Web: http://life.xmu.edu.cn/adhanlab/