I have had protein crystals (so very high protein concentration) that turn into gummy bear-like objects, where instead of crumbling they are like, well, a gummy bear or a piece of rubber. I attributed it to oxidation or other chemical ageing processes. I am sure others will have suggestions for preventing this.
On Tue, Aug 30, 2011 at 11:31 AM, aidong <a...@xmu.edu.cn> wrote: > Dear Buddies, > > Sorry for bothering you with an off-ccp4 question. We recently are > experiencing a very strange phenomena. A couple of protein preps with > reasonably high concentration (10-20mg/ml) become a jelly after storages for > overnight or a couple of days at 4C. All of them have been purified by gel > filtration. Some of these proteins behave like this from very first preps > but some of them had been very kind to us previously. We have googled > extensively in CCP4BB and www but it appears this only happens to us. It > would be highly appreciated that you could exchange their experiences or > provide your suggestions. > > Aidong Han, Ph.D > > Department of Biomedical Sciences > School of Life Sciences > Xiamen University > Xiamen, Fujian 361005 > China > Phone: 0592-218-8172 (O) > 0592-218-8173 (L) > Web: http://life.xmu.edu.cn/**adhanlab/ <http://life.xmu.edu.cn/adhanlab/> >
