Gamma delta resolvase catalytic domain stock solutions used to make a nice clear jelly at 4 degrees, but it was perfectly reversible by warming the sample to room T. In fact, one mutant crystallized in the stock tube after a few trips in and out of the fridge. The crystals didn't diffract very well (so we never published that), but it was a fun way to grow them. Phoebe
===================================== Phoebe A. Rice Dept. of Biochemistry & Molecular Biology The University of Chicago phone 773 834 1723 http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 http://www.rsc.org/shop/books/2008/9780854042722.asp ---- Original message ---- >Date: Tue, 30 Aug 2011 23:31:20 +0800 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of aidong ><a...@xmu.edu.cn>) >Subject: [ccp4bb] Protein preps become a jelly >To: CCP4BB@JISCMAIL.AC.UK > >Dear Buddies, > >Sorry for bothering you with an off-ccp4 question. We recently are >experiencing a very strange phenomena. A couple of protein preps with >reasonably high concentration (10-20mg/ml) become a jelly after >storages for overnight or a couple of days at 4C. All of them have >been purified by gel filtration. Some of these proteins behave like >this from very first preps but some of them had been very kind to us >previously. We have googled extensively in CCP4BB and www but it >appears this only happens to us. It would be highly appreciated that >you could exchange their experiences or provide your suggestions. > >Aidong Han, Ph.D > >Department of Biomedical Sciences >School of Life Sciences >Xiamen University >Xiamen, Fujian 361005 >China >Phone: 0592-218-8172 (O) > 0592-218-8173 (L) >Web: http://life.xmu.edu.cn/adhanlab/
