Dear ccp4, I am refining one structure at low resolution (3.5 A). I solved the structure in space group P622 by molecular replacement. The Rw/Rf after some cycle of rigid body refinement in Refmac5 is 34/37. But when I am trying to refine with Restrain refinement in Refmac the Rw is going down but the Rf is going up. This same happens with the phenix refinement. The protein is quite big 520 residues and one molecule is present in the crystal asymmetric unit. The maps are also not so bad except some region.
Please give me suggestion, what should I have to care during low resolution refinement? or is there any other tricks for this types of data? Thanks in advance. Md. Munan Shaik PhD Student Department of Biotehnology School of Bioscience and Biotechnology via G. Colombo 03 Padova 35131, Italy Mobile: 00393275671896 E-mail: munanbt2...@yahoo.com munan.sh...@unipd.it
