Are you sure of the space group? P622 is much rarer than P6(x)22 where x > 0 Phil
On 14 Sep 2011, at 15:34, Md Shaik wrote: > Dear ccp4, > > I am refining one structure at low resolution (3.5 A). I solved the structure > in space group P622 by molecular replacement. The Rw/Rf after some cycle of > rigid body refinement in Refmac5 is 34/37. But when I am trying to refine > with Restrain refinement in Refmac the Rw is going down but the Rf is going > up. This same happens with the phenix refinement. The protein is quite big > 520 residues and one molecule is present in the crystal asymmetric unit. The > maps are also not so bad except some region. > > Please give me suggestion, what should I have to care during low resolution > refinement? or is there any other tricks for this types of data? > > Thanks in advance. > > > Md. Munan Shaik > PhD Student > Department of Biotehnology > School of Bioscience and Biotechnology > via G. Colombo 03 > Padova 35131, Italy > Mobile: 00393275671896 > E-mail: munanbt2...@yahoo.com > munan.sh...@unipd.it >
