Hi, did you use refinement strategy suitable for low (3.5A) resolution (secondary structure restraints, Ramachandran plot restraints, proper ADP parameterization, SA, etc.... etc...)?
If you send me the data and model files off-list I will have a look. Pavel On Wed, Sep 14, 2011 at 7:34 AM, Md Shaik <munanbt2...@yahoo.com> wrote: > Dear ccp4, > > I am refining one structure at low resolution (3.5 A). I solved the > structure in space group P622 by molecular replacement. The Rw/Rf after some > cycle of rigid body refinement in Refmac5 is 34/37. But when I am trying to > refine with Restrain refinement in Refmac the Rw is going down but the Rf is > going up. This same happens with the phenix refinement. The protein is quite > big 520 residues and one molecule is present in the crystal asymmetric unit. > The maps are also not so bad except some region. > > Please give me suggestion, what should I have to care during low resolution > refinement? or is there any other tricks for this types of data? > > Thanks in advance. > > > Md. Munan Shaik > PhD Student > Department of Biotehnology > School of Bioscience and Biotechnology > via G. Colombo 03 > Padova 35131, Italy > Mobile: 00393275671896 > E-mail: munanbt2...@yahoo.com > munan.sh...@unipd.it > >
