On 2011-06-16 13:06, Filip Van Petegem wrote:
Even if evaporation is not an issue, one has to take pipetting errors into account when dealing with small volumes. The relative error on 1-2ul is a lot bigger than on 50ul.
True, but the nanodrop works independent of volumes, since it has a fixed pathlength. 1ul loaded will give the same result as 2ul loaded.
hth -Bjørn -- Bjørn Panyella Pedersen Macromolecular Structure Group Dept. of Biochemistry and Biophysics University of California, San Francisco
