Dear All I have a difficulty to crystallize my membrane protein(his tagged). I got the salt crystals from many different screening conditions. The protein is in PBS buffer and 130mM salt with 0.1% SDS. I store It in the 4 degree although it forms the milk solution. The solution is getting clear in the room temperature within 5 mins when I set up the trays.
I would like to take any advice. I would appreciate your input. Best Min
