It might be SDS precipitation. Although 0.1% SDS is generally considered not high enough to precipitate at 4 degree, it might interact with other components in your solution to form even less soluble material.
Nian On Fri, May 20, 2011 at 9:36 AM, WEI MIN <butiany...@gmail.com> wrote: > Dear All > > I have a difficulty to crystallize my membrane protein(his tagged). I got > the salt crystals from many different screening conditions. The protein is > in PBS buffer and 130mM salt with 0.1% SDS. I store It in the 4 degree > although it forms the milk solution. > The solution is getting clear in the room temperature within 5 mins when I > set up the trays. > > I would like to take any advice. I would appreciate your input. > > Best > > Min >
