try to change ur buffer instead of PBS u can use Tris or HEPES. In PBS buffer u will get salt crystals in many condition becoz of phopshaphate........ try to use nonionic detergents in ur soluton ..........
On Fri, May 20, 2011 at 8:29 PM, Nian Huang <huangn...@gmail.com> wrote: > It might be SDS precipitation. Although 0.1% SDS is generally considered > not high enough to precipitate at 4 degree, it might interact with other > components in your solution to form even less soluble material. > > Nian > > > On Fri, May 20, 2011 at 9:36 AM, WEI MIN <butiany...@gmail.com> wrote: > >> Dear All >> >> I have a difficulty to crystallize my membrane protein(his tagged). I got >> the salt crystals from many different screening conditions. The protein is >> in PBS buffer and 130mM salt with 0.1% SDS. I store It in the 4 degree >> although it forms the milk solution. >> The solution is getting clear in the room temperature within 5 mins when I >> set up the trays. >> >> I would like to take any advice. I would appreciate your input. >> >> Best >> >> Min >> > > -- Vandana Kukshal Postdoc Fellow structure biology group international Center For Genetic Engineering and Biotechnolgy India
