[ccp4bb] **Possible spam**Re: Low resolution refinement

[Kay Diederichs]

Hi Joane,




HTH,

Kay
--
Kay Diederichs                http://strucbio.biologie.uni-konstanz.de
email: kay.diederi...@uni-konstanz.de    Tel +49 7531 88 4049 Fax 3183
Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz

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since you're using NCS I'd like to add that this is indeed a good strategy, but you should make sure that you should probably not restrain the B-factors of the well-defined domain and the not-so-well-defined domains to be equal - they do differ and the refinement should probably not change that! So I'd say: adjust the weights such that the coordinates are NCS-restrained, but not the B-factors (or only a little). Use R-free to find the right values for the restraints.

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