Dear Dr Ku, It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg and 1mM EDTA.
And I want to try other affinity columns, just don't know what resin would be appropriate. Sincerely, Xuan Yang 2009/9/3 Shao-Yang Ku <s...@embl-hamburg.de> > Can you put a 6His-tag on your protein and add some Ni-NTA beads to your > solution to capture (and concentrate) the properly folded molecules? > > > Quoting "Xuan Yang" <pattisy...@gmail.com>: > > Dear All, >> >> I am working on protein refolding via dialysis in large volumn >> (typically 2~4 litters). It was problematic when I wanted to concentrate >> the >> solution to at least less than 500ml. If you know any device appropriate >> for >> such task, please help me out:) >> >> Thanks in advance! >> >> Sincerely, >> >> Xuan Yang >> >> >
