Hi,
We had success using a testosterone hemisuccinate derivative which is
much more soluble.
For details, see: Drevelle et al; J Mol Biol. <javascript:AL_get(this,
'jour', 'J Mol Biol.');> 2006 Apr 28;358(2):455-71
Hope this helps!!
Marc
KUMARASWAMI MUTHIAH a écrit :
Anybody tried to cocrystallize the protein-progesterone or estrogen
complexes, if so how do you go about the solubility of these
compounds? Progesterone is only soluble in 50% chloroform or 100% DMSO
and the dilution of this stock is not possible as chloroform falls out
of solution. Lots of papers out there used soaking with progesterone
or expressed the protein in the presence of progesterone. Any
suggestions would be appreciated.
Thanks
------------------------------------------------------------------------
Rediscover Hotmail®: Now available on your iPhone or BlackBerry Check
it out.
<http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009>
--
Marc Graille, PhD
Yeast Structural Genomics, IBBMC CNRS UMR8619 Bat 430 Universite Paris Sud
91405 Orsay Cedex
NEW PHONE NUMBER
Tel: (33) 1 69 15 31 57
Fax: (33) 1 69 85 37 15
