Hi,
it worked very nice for me in 1 out of 1 case where I tried it :-).
Very well diffracting crystals (1.8 Ang), rather small protein 20
kDa, 50 %solvent content, 1 mol/ASU. 20-30 s soak in 0.5 M NaBr
resulted in 6 nice ordered sites.
It was crucial for us to collect a 3 wavelength MAD data set. A SAD
data set (using just the peak, even if with high redundancy ) was not
enough to obtain traceable electron density map, even-though
one could distinguish clearly protein boundaries and solvent channels.
Good luck
Ale
On 31 Mar 2009, at 18:19, tat cheung cheng wrote:
Hi all
I am now trying to do bromide soaking, but i am not really sure does
the bromide atom enter my crystal. So is there any signs that
indicate the entry of bromide atom? e.g. does the space group, cell
dimension change? or just nothing change, and the bromide atom just
get in?
Thanks very much.
T.C. Cheng
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