I'd like to remind folks here that if one's crystals are sturdy enough to survive halide soaking, they're *probably* also sturdy enough to live through covalent iodination. Iodination is easy to set up and if it does work out - one gets awesome quality derivatives with multiple sites (as long as there are enough Tyrosines in the protein). The drawback is that iodination can and often does kill the crystal - which is the reason for my earlier comment regarding crystal machismo/resilience.
If you want to try it, I would be happy to supply an easy protocol that worked for me several times :) Artem > You always get the entry of Bromide into crystal by quick soaking, > because it does not require the incorporation of Bromide into the > protein. But whether the signal is good enough for phasing is another > story. You have to collect the full data set to know the answer. > > Nian > > 2009/3/31 tat cheung cheng <theif...@yahoo.com.hk>: >> Hi all >> >> I am now trying to do bromide soaking, but i am not really sure does the >> bromide atom enter my crystal. So is there any signs that indicate the >> entry of bromide atom? e.g. does the space group, cell dimension change? >> or just nothing change, and the bromide atom just get in? >> Thanks very much. >> >> T.C. Cheng >> >> >> Yahoo!»´ä´£¨Ñºô¤W¦w¥þ§ð²¤¡A±Ð§A¦p¦ó¨¾½d¶Â«È! ½Ð«e©¹ >> http://hk.promo.yahoo.com/security/ ¤F¸Ñ§ó¦h! >> >
