Would you put the iodide protocol on the wiki? If not, please send it to me
directly.
JPK
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
----- Original Message -----
From: <ar...@xtals.org>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Tuesday, March 31, 2009 1:07 PM
Subject: Re: [ccp4bb] Halide soaking
I'd like to remind folks here that if one's crystals are sturdy enough to
survive halide soaking, they're *probably* also sturdy enough to live
through covalent iodination. Iodination is easy to set up and if it does
work out - one gets awesome quality derivatives with multiple sites (as
long as there are enough Tyrosines in the protein). The drawback is that
iodination can and often does kill the crystal - which is the reason for
my earlier comment regarding crystal machismo/resilience.
If you want to try it, I would be happy to supply an easy protocol that
worked for me several times :)
Artem
You always get the entry of Bromide into crystal by quick soaking,
because it does not require the incorporation of Bromide into the
protein. But whether the signal is good enough for phasing is another
story. You have to collect the full data set to know the answer.
Nian
2009/3/31 tat cheung cheng <theif...@yahoo.com.hk>:
Hi all
I am now trying to do bromide soaking, but i am not really sure does the
bromide atom enter my crystal. So is there any signs that indicate the
entry of bromide atom? e.g. does the space group, cell dimension change?
or just nothing change, and the bromide atom just get in?
Thanks very much.
T.C. Cheng
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