Here's another paper you might want to have a look at:
Carson et al. His-tag impact on structure. Acta Crystallogr D Biol
Crystallogr (2007) vol. 63 (Pt 3) pp. 295-301
and here's the Structural Genomics paper (or one of them, I believe
there was another one which I originally thought of):
Liu et al. The high-throughput protein-to-structure pipeline at SECSG.
Acta Crystallogr D Biol Crystallogr (2005) vol. 61 (Pt 6) pp. 679-84
Jürgen
On 14 Nov 2008, at 09:34, Melanie Adams wrote:
Also check out the following reviews:
Protein Sci. 2003 July; 12(7): 1313–1322.
Crystal structures of fusion proteins with large-affinity tags
Douglas R. Smyth, Marek K. Mrozkiewicz, William J. McGrath, Pawel
Listwan, and Bostjan Kobe1
Gene Volume 281, Issues 1-2, 27 December 2001, Pages 1-9
Structural analysis of regulatory protein domains using GST-fusion
proteins
Yong Zhan, Xi Song and G. Wayne Zhou
Cheers
Mel
-----Original Message-----
From: CCP4 bulletin board on behalf of Artem Evdokimov
Sent: Thu 11/13/2008 5:50 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] SUMMARY - crystallization of proteins with His-
tag and/or c-myc tags
There are quite a few MBP fusions in the PDB. Just search using MBP
sequence
and you will get (among others):
1A7L
1HSJ
1IUD
1MG1
1MH3
1NMU
1R6Z
And so on...
Artem
-----Original Message-----
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf
Of David
M Shechner
Sent: Thursday, November 13, 2008 8:32 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] SUMMARY - crystallization of proteins with His-
tag
and/or c-myc tags
> I have a more general question that's come up in discussion with
former
> colleagues: what's the largest tag that has been co-crystallized
with the
> target protein? I'm specifically wondering about MBP - we've
encountered
> several proteins that would express decently (and, apparently,
correctly
> folded) with a His-MBP tag but crashed out of solution when the
tag was
> cleaved. But I don't think anyone ever tried leaving the tag on for
> crystallization trials. Or what about GST?
Hey, Nat, (et. al.),
Actually, I do know of one example using MBP. Jamie Williamson's
lab used
an
MBP fusion with the L30e protein in both their crystallographic and
NMR
solution of its complex with RNA. Check out the following, as well as
references therein:
Chao JA, Williamson JR. (2004) Joint X-ray and NMR refinement of the
yeast
L30e-mRNA complex. Structure.2004 Jul;12(7):1165-76.
Cheers,
D.S.
-
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
