Also check out the following reviews: Protein Sci. 2003 July; 12(7): 13131322. Crystal structures of fusion proteins with large-affinity tags Douglas R. Smyth, Marek K. Mrozkiewicz, William J. McGrath, Pawel Listwan, and Bostjan Kobe1
Gene Volume 281, Issues 1-2, 27 December 2001, Pages 1-9 Structural analysis of regulatory protein domains using GST-fusion proteins Yong Zhan, Xi Song and G. Wayne Zhou Cheers Mel -----Original Message----- From: CCP4 bulletin board on behalf of Artem Evdokimov Sent: Thu 11/13/2008 5:50 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] SUMMARY - crystallization of proteins with His-tag and/or c-myc tags There are quite a few MBP fusions in the PDB. Just search using MBP sequence and you will get (among others): 1A7L 1HSJ 1IUD 1MG1 1MH3 1NMU 1R6Z And so on... Artem -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of David M Shechner Sent: Thursday, November 13, 2008 8:32 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] SUMMARY - crystallization of proteins with His-tag and/or c-myc tags > I have a more general question that's come up in discussion with former > colleagues: what's the largest tag that has been co-crystallized with the > target protein? I'm specifically wondering about MBP - we've encountered > several proteins that would express decently (and, apparently, correctly > folded) with a His-MBP tag but crashed out of solution when the tag was > cleaved. But I don't think anyone ever tried leaving the tag on for > crystallization trials. Or what about GST? Hey, Nat, (et. al.), Actually, I do know of one example using MBP. Jamie Williamson's lab used an MBP fusion with the L30e protein in both their crystallographic and NMR solution of its complex with RNA. Check out the following, as well as references therein: Chao JA, Williamson JR. (2004) Joint X-ray and NMR refinement of the yeast L30e-mRNA complex. Structure.2004 Jul;12(7):1165-76. Cheers, D.S.
