This has been reviewed, although a few years ago now. In most cases the linker between the fusion tag and the protein of interest had to be shortened and engineered. See http://www.ncbi.nlm.nih.gov/pubmed/12824478?ordinalpos=2&itool=EntrezSystem2 .PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSu m
Some recent clever examples of using large fusion tags involve lysozyme in beta-adrenergic receptor and joining an LRR domain from a different protein to the ectodomain from TLRs. Bostjan On 14/11/08 11:50 AM, "Artem Evdokimov" <[EMAIL PROTECTED]> wrote: > There are quite a few MBP fusions in the PDB. Just search using MBP sequence > and you will get (among others): > > 1A7L > 1HSJ > 1IUD > 1MG1 > 1MH3 > 1NMU > 1R6Z > > And so on... > > Artem > > -----Original Message----- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of David > M Shechner > Sent: Thursday, November 13, 2008 8:32 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] SUMMARY - crystallization of proteins with His-tag > and/or c-myc tags > >> I have a more general question that's come up in discussion with former >> colleagues: what's the largest tag that has been co-crystallized with the >> target protein? I'm specifically wondering about MBP - we've encountered >> several proteins that would express decently (and, apparently, correctly >> folded) with a His-MBP tag but crashed out of solution when the tag was >> cleaved. But I don't think anyone ever tried leaving the tag on for >> crystallization trials. Or what about GST? > > Hey, Nat, (et. al.), > > Actually, I do know of one example using MBP. Jamie Williamson's lab used > an > MBP fusion with the L30e protein in both their crystallographic and NMR > solution of its complex with RNA. Check out the following, as well as > references therein: > > Chao JA, Williamson JR. (2004) Joint X-ray and NMR refinement of the yeast > L30e-mRNA complex. Structure.2004 Jul;12(7):1165-76. > > Cheers, > D.S. --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Molecular and Microbial Sciences and Institute for Molecular Bioscience Cooper Road University of Queensland Brisbane, Queensland 4072 Australia Phone: +61 7 3365 2132 Fax: +61 7 3365 4699 E-mail: [EMAIL PROTECTED] URL: http://profiles.bacs.uq.edu.au/Bostjan.Kobe.html Office: Building 76 Room 452 Notice: If you receive this e-mail by mistake, please notify me, and do not make any use of its contents. I do not waive any privilege, confidentiality or copyright associated with it. Unless stated otherwise, this e-mail represents only the views of the Sender and not the views of The University of Queensland.
