Many years ago, someone from UMass told me that he has crystallized many proteins with GST-tag and believed that dimerization of GST helped crystallization. I don't remember his name. --Chun
_____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Nathaniel Echols Sent: Thursday, November 13, 2008 5:01 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] SUMMARY - crystallization of proteins with His-tag and/or c-myc tags On Thu, Nov 13, 2008 at 9:47 AM, iulek <[EMAIL PROTECTED]> wrote: But..., no one commented about c-myc tags. As I expected, there is no experience with this one yet - ? I have a more general question that's come up in discussion with former colleagues: what's the largest tag that has been co-crystallized with the target protein? I'm specifically wondering about MBP - we've encountered several proteins that would express decently (and, apparently, correctly folded) with a His-MBP tag but crashed out of solution when the tag was cleaved. But I don't think anyone ever tried leaving the tag on for crystallization trials. Or what about GST? The closest example I can think of is T4 lysozyme embedded in a cytoplasmic loop of the beta-adrenergic receptor, but that's nowhere near as trivial as a generic expression vector. (And even T4 lysozyme is less than half the size of MBP.)
