Hi Sabine, Your problem might be an issue of the PDB format. I had troubles using a DNA model in coot which was refined in cns (3 letter code). When I converted it to the single letter notation everything was fine in coot/refmac.
Tschüß, christian Sabine Schneider wrote: > Hi everyone, > > I am working on Protein-DNA complex and am struggling to generate a > dictionary with the right bonds/restraints for a the ds DNA of 15nt > length with 1nt overhang and a lesion. How do I link the nucleotides > together and set the right restraints in the bit with the lesion? Tried > to read in a bit of DNA into library scatcher to get an idea what I have > to do, but failed. Also couldn't find any example cif-files. > > Thanks for your help in advance! > > Sabine > _______________________________________________________________________ Dr. Christian Biertümpfel Laboratory of Molecular Biology NIDDK/National Institutes of Health phone: +1 301 402 4647 9000 Rockville Pike, Bldg. 5, Rm. B1-03 fax: +1 301 496 0201 Bethesda, MD 20892-0580 USA _______________________________________________________________________
