Hi,
I would suggest that you may harvest the crystals at different time, and
then mount them and test at room temperture.
Our lab has the experience that large crystals always have lower resolution
than medium size(0.1-0.2mm). So try and good luck.
deliang
----- Original Message -----
From: "Juergen Bosch" <[EMAIL PROTECTED]>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Wednesday, January 24, 2007 12:29 PM
Subject: Re: [ccp4bb] Fancy crystal, poor diffraction
Hi Tiancen,
nobody so far has suggested to mount the smallest crystals, 0.4x0.4x0.3
seems to be pretty large, this also means that the freezing procedure is
longer. Then the other question is do you freeze in the Cryostream or by
plunging into LN2, I'd prefer the latter technique.
Good luck,
Juergen
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
