Hi,
You might want to look at
Berejnov, V., Husseini, N. S., Alsaiedc, O. A. & Thorne, R. E.
(2006). J. Appl. Cryst. 39, 244-251.
Regards,
John
John Rose Ph.D.
Associate Professor
B204B, The Fred C. Davison Life Sciences Complex
120 Green Street
Department of Biochemistry and Molecular Biology
University of Georgia
Athens, GA 30602-7229
=========================================
Phone: 706-542-1750
Fax: 706-542-3077
On Jan 24, 2007, at 3:29 PM, Juergen Bosch wrote:
Hi Tiancen,
nobody so far has suggested to mount the smallest crystals,
0.4x0.4x0.3
seems to be pretty large, this also means that the freezing
procedure is
longer. Then the other question is do you freeze in the Cryostream
or by
plunging into LN2, I'd prefer the latter technique.
Good luck,
Juergen
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002