>> Have you tried DTT (or another way of keeping things in a reducing >> environment)? This might help keep your disulfides (and hopefully >> protein) happy. > > > Surely the addition of a _reducing_ agent would _reduce_ your disulphides > - > i.e. break them.
Whoops...sorry, I guess I've got zinc sites on the brain. Thanks for the correction, Pete Pete Meyer Fu Lab BMCB grad student Cornell University
