[ccp4bb] technical question

Hi everbody, I want to replace a particular methionine in a pdb with methionine sulfoxide( an oxidized form of methionine). Can body please tell me how to do this? I am familiar with pymol, chimera and coot software. -- *Amit Gaur* *Post Doctoral ResearcherThomas Jefferson University* *1020

Re: [ccp4bb] technical question

Thanks every one. I tried this and worked pretty well. On Fri, Nov 29, 2019 at 4:42 PM Robbie Joosten wrote: > Or use the residue name MHO if SME has the wrong hand around the sulfur. > > Cheers, > Robbie > > On 29 Nov 2019 21:47, Paul Emsley wrote: > > On 29/11/201

[ccp4bb] Homology modeling

Hi All, I am trying to generate a model using comparative modeling. Can anybody suggest the quality of the model based on procheck summary. +--<<< P R O C H E C K S U M M A R Y >>>--+ |

[ccp4bb] hydrophobic forces

Hi All, I have a question that, I have a loop region with hydrophilic residues(TNETRERE) that lie close to methionine. Can, repulsive forces may occur because between methionine and hydrophilic residues? -- *Dr. Amit Gaur* *Post Doctoral Researcher* *Thomas Jefferson University

[ccp4bb] Hydrophobic/ vander waal forces

propensity to form these interactions? Thank you, -- *Dr. Amit Gaur* *Research AssociateThomas Jefferson University* *Department of Translational medicine* *1020, Locust Street, Jefferson Alumni Hall* *Philadelphia, PA 19107

[ccp4bb] Dimer in SDS-PAGE

tetramer which is common oligomerizaton of potassium channel family with GYG motif. Can any body suggest what should I do in this case? Thanks and regards, -- *Dr. Amit Gaur* *Post Doctoral ResearcherPI: Dr. Ji-Fang ZhangThomas Jefferson University* *1020, Locust Street, Suite 418

[ccp4bb] Co-Crystallization with drug molecule

. Thanks, *Dr. Amit Gaur,* *Research Scientist* *Center for Biotechnology and **Interdisciplinary Studies,* *Rensselaer Polytechnic Institute,* *1623 15th Street, Troy, NY, 12180* To unsubscribe from the CCP4BB list, click the

Re: [ccp4bb] Crystallization of SARS-CoV-2 3CLpro and Mutants – Seeking Advice on Crystal Optimization

Hi Mohit, I was able to get crystals of C111S-PLpro using microbatch under oil method in crystal screen of hampton research. Protein concentration was around 10 mg/ml. Crystals diffracted to 2.5 A at NSLS-II. *Dr. Amit Gaur, * *Department of Biomedical Science,* *College of Medicine,* *Florida