Hi everyone, I am trying to crystallize a protein with a drug molecule. The protein concentration is 15.5 mg/ml, the drug stock concentration is 10 mM, and the drug is dissolved in DMSO. I am adding the drug to a final concentration of 1 mM in 100 ul of protein, and the DMSO volume is 10 ul for Co-crystallization. I want to know how much DMSO is permissible during co-crystallization with the drug and if DMSO can poison crystal formation. I have not been successful in getting crystals with inhibitors till now, but I obtained crystals of protein without DMSO, and those diffracted to 2.5A.
Thanks, *Dr. Amit Gaur,* *Research Scientist* *Center for Biotechnology and **Interdisciplinary Studies,* *Rensselaer Polytechnic Institute,* *1623 15th Street, Troy, NY, 12180* ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
