Dear All,
3D structure modeling server I-TASSER predicts a binding site for NADPH and
I want to test this prediction. What would be the nice quick way to tell
whether this protein bind NADPH or not, when I have a lot of recombinant
protein?
Sincerely,
Xuan Yang
Dear Jacob,
Nanodrop ultrafiltration sounds really fancy to me. I am afraid that I have
no access to such equipment yet.
Thanks for letting me know about this new technology.
Sincerely,
Xuan Yang
2010/8/4 Jacob Keller
> I like nanodrop ultrafiltration:
>
> concentrate your prote
alaria Research Institute
> 615 North Wolfe Street, W8708
> Baltimore, MD 21205
> Phone: +1-410-614-4742
> Lab: +1-410-614-4894
> Fax: +1-410-955-3655
> http://web.mac.com/bosch_lab/
>
> On Aug 4, 2010, at 4:10, Xuan Yang wrote:
>
> > Dear All,
> >
>
).
However, considering the instability of Fe(II) in solution, the design
still seemed problematic.
Sincerely,
Xuan Yang
National Laboratory of Biomacromolecules and
Center for Infection and Immunity,
Institute of Biophysics,
Chinese Academy of Sciences,
Room 1617, 15 DaTun Road,Chaoyang District
stoichiometry increaed to 1:1 in the C-terminal part. The result lead me
to focus on Zn instead of Fe. But I still want to confirm the idea.
Matallo biochemistry was exactly what I dreamed to do.
Sincerely,
Xuan Yang
2007/8/6 Guenter Fritz
> Hi Xuan,
> I guess your protein is not an E.coli p
s?
> >>
> >> Specifically, now I want to compare the binding efficiency on various
> >> IMAC, i.e. 50mM ZnSO4, FeSO4, Fe2(SO4)3, NiSO4(control), or
> >> CuSO4(control). However, considering the instability of Fe(II) in
> >> solution, the design still see
!
Sincerely,
Xuan Yang
2009/8/27 James Stroud
> Just try crystallizing it. What is a crystal but a "massive aggregate"?
> That they are still soluble and active is great news.
>
> As a grad student, I had a similar phenomenon with an early project. I
> showed a gel in group me
crease the solubiltiy and homogeneity, whichmight be helpful too.
Good luck!
Xuan Yang
2009/8/27 ose toyoyuki
>
> Dear all,
>
> This is a question on how to cope with the protein that seems to form
> massive aggregates in solution but enzymatically active.
>
> I'm wo
Dear All,
I am working on protein refolding via dialysis in large volumn
(typically 2~4 litters). It was problematic when I wanted to concentrate the
solution to at least less than 500ml. If you know any device appropriate for
such task, please help me out:)
Thanks in advance!
Sincerely,
Xuan
Dear Dr Ku,
It was a wonderful idea! However, the refolding buffer contained 500mM L-Arg
and 1mM EDTA.
And I want to try other affinity columns, just don't know what resin would
be appropriate.
Sincerely,
Xuan Yang
2009/9/3 Shao-Yang Ku
> Can you put a 6His-tag on your protein and
Dear all,
I have collected a dataset on a Saturn 944HG CCD detector, and I want to
process the data on HKL2000. The detector is not found in the list of the
HKL2000 pop-up window in the beginning, what should I do to make the
HKL2000 package recognize my dataset?
I'm a computer dummy, so would y
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