Re: [ccp4bb] Na acetate as purification buffer

to know the range of different buffers and their long term shelf life and stability. http://www.sigmaaldrich.com/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html I hope it helps you figure out the optimum pH conditions for you target protein. Best, Santosh

Re: [ccp4bb] weird--No protein expression using pET30a

) for expressing toxic and membrane proteins. If nothing helps then to shuttle the gene into yeast (*Pichia pastoris*), insects or mammalian expression system. I hope it helps and wish you all the best. Have a great weekend. Santosh Hodawadekar, PhD http://www.linkedin.com/in/shodawadekar/ On

Re: [ccp4bb] Please remove my email from the list shodawade...@gmail.com

Hello, I have been using this portal for past eight years, learned many intricate aspects of crystallography. now that I have changed my career please make sure to remove me from the list. I wish you all wonderful years and best wishes. Sincerely, Santosh

Re: [ccp4bb] how to combine the experimental phase and molecular replacement phase

model phase, experimental phasing, phase combination, density modification, model building and refinement of the built model. Iteratively it improves phases and complete your model as much as possible. Best Santosh Quoting "Jiamu Du" : Dear All, I am now working on a low

Re: [ccp4bb] Help needed in solving a MAD dataset

Dear All, Not only for beginners, but also for experts who want to solve structures in hurry at the beamline and who want to complete model starting from X-ray data at resolution better than 3.0 A resolution. Santosh Quoting "Ulrich Zander" : Hi Qing Lu, try Autorick

[ccp4bb] DANO from PDB

Hi all, Does anybody have a program which can calculate anomalous differences or F+ and F- from a refined structure at given wavelength and resolution ? Thanks Santosh Santosh Panjikar, Ph.D. [EMAIL PROTECTED] Staff Scientist EMBL Hamburg outstation http

[ccp4bb] Auto-Rickshaw: the EMBL-HH automated crystal structure determination platform

detailed information visit http://www.embl-hamburg.de/Auto-Rickshaw The Auto-Rickshaw server is freely accessible to users who have the necessary software licences. Academic usage requires free of charge on-online registration at the same web site. Further inquiries can be made to Santosh Panjikar

Re: [ccp4bb] Best compounds for heavy atom soaks

protein, higher the chance of Xe/Kr binding. best Santosh Santosh Panjikar, Ph.D. Scientist Australian Synchrotron 800 Blackburn Road Clayton VIC 3168 Australia Ph: +61-4-67770815 (mobile) +61-3-85404276 (office)

Re: [ccp4bb] a challenge

Rickshaw used SHELXC/D/E and ARP/wARP/REFMAC5 to get the partially refined model (Rfree below 30%) . Cheers Santosh Santosh Panjikar, Ph.D. Scientist Australian Synchrotron 800 Blackburn Road Clayton VIC 3168 Australia Ph: +61-4-67770851 From: CCP4 bu

Re: [ccp4bb] Problem in running ARP_WARP in CCP4i

try tcsh auto_tracing.sh datafile ../psp.mtz Santosh Panjikar, Ph.D. Scientist Australian Synchrotron 800 Blackburn Road Clayton VIC 3168 Australia Ph: +61-4-67770815 (mobile) +61-3-85404276 (office) From: CCP4 bulletin board [CCP4BB