Dear All,
We are working with proteins with a lot of surface hydrophobicity and
hence solubility is a big issue. We so far have tried expressing them as fusion
proteins.The strategy has yielded soluble protein but most of the protein
elutes in the void volume on a gel-filtration colum.
Dear all,
We work with lipid droplet binding proteins in our group. These
proteins behave more like membrane associated proteins, have a very hydrophobic
surface and require detergents right from solubilization to crytallization.
We use 1% DDM for solubilization, 0.03% DDM t
Dear All,
I have got initial crystals in a condition with PEG 1000. The PEG 1000
stock we had in our lab was rock solid and when i heated it to about 50 degrees
for 15 to 20 minutes it became a solution. We thought the compound has got out
dated or something like that and bought a bran
Hello all,
We co-crystallized an inactive variant of our enzyme in the
presence of substrate and have determined the structure at 1.85A.
Now, we want to validate the fitting of the ligand into the
electron density. We tried validating using the difference map (2Fo-Fc)
gt;>>>>Although the
Twilight program can only look at deposited PDB entries, the
>>>>>tips
about ligand validation in the paper are very useful. I suggest
you
>>>>>start from there.
>>>>>You can use EDSTATS in CCP4 to
