Hello all, We co-crystallized an inactive variant of our enzyme in the presence of substrate and have determined the structure at 1.85A.
Now, we want to validate the fitting of the ligand into the electron density. We tried validating using the difference map (2Fo-Fc) after refining the structure without the ligand. But, it is still a bit inconclusive if the density fits the ligand. It would be very kind to know if there are tools for validating this electron density. We were excited about twilight but turns out it can only be used with deposited structure. We will appreciate your help and suggestions. Many thanks, Srinivasan
