Dear All, We are working with proteins with a lot of surface hydrophobicity and hence solubility is a big issue. We so far have tried expressing them as fusion proteins.The strategy has yielded soluble protein but most of the protein elutes in the void volume on a gel-filtration colum.
This brings me to my question, "Are there vectors which have an inactive lysozyme fusion tag, which could be used for expression in E.coli?". Many thanks to all in anticipation, Vasan
