i get the same error on OS X 10.5.2 and 10.4.11:
>sfcheck -f refmac2.mtz -m refmac2.pdb -out y -nomit 2 -map
the error:
Open failed: Unit: 11, File: sfcheck_scr.dat (logical: sfcheck_scr.dat)
Last system error message: Bad file descriptor
C
TED]> wrote:
> On 4 Apr 2008, at 01:36, Michael Giffin wrote:
>
>
> > i get the same error on OS X 10.5.2 and 10.4.11:
> >
> >
> > > sfcheck -f refmac2.mtz -m refmac2.pdb -out y -nomit 2 -map
> > >
> >
> > the error:
> > ---
gions of the
> vector onto the primers which also generate the initial PCR product. I plan
> to proceed with my insert which is ~ 2kb and am curious to get some feedback
> if you have successfully cloned inserts > 1.5kb using the above strategy.
>
> Many thanks.
> Raji
>
Micha
name, concentration, and full spectra.
It is expensive, but so are good cuvettes.
Mike
Michael Giffin
The Scripps Research Institute
Department of Molecular and Experimental Medicine
10550 North Torrey Pines Road, MEM-131
La Jolla, CA 92037
email: [EMAIL PROTECTED]
lab: 858-784-7758
On Thu
the solution needs to be
> sufficiently dilute so that the chance of molecules "shadowing" one another
> is negligible. Isn't this condition violated for concentrated solutions
> (even with short path lengths)?
> Pat
> On 4 Dec 2008, at 1:27 P
nality are you looking for?
Mike
Michael Giffin
The Scripps Research Institute
Department of Molecular and Experimental Medicine
10550 North Torrey Pines Road, MEM-131
La Jolla, CA 92037
email: mjgif...@scripps.edu
lab: 858-784-7758
On Wed, Jan 28, 2009 at 7:02 AM, Mark Collins
wrote:
>
