Re: [ccp4bb] AW: Going back to Coot 0.8

Dear Paul, we have the same experience in our lab. Personally, I did would not like to judge here, as so far, I did not have had enough time to get into the new RSR of coot 0.9.x by myself. But many colleagues did not like the new refinement module maybe just as they are used to the method in

Re: [ccp4bb] AW: Going back to Coot 0.8

nt of coot and I'm really grateful that coot is available. All the best, Georg Am 08.09.20 um 17:44 schrieb Paul Emsley: On 08/09/2020 16:25, Georg Zocher wrote: we have the same experience in our lab. What experience is that? I am still in the dark about you think is now worse.

Re: [ccp4bb] AW: Going back to Coot 0.8

er is harder to except than. But that's ok as this keeps one flexible. I hope, I will find the time soon and get used to it as much as whatever "the thing labelled as coffee" was;-) Thank you all for your contributions, Georg Cheers, Tim On Wed, 9 Sep 2020 17:22:27 +0200 Georg Z

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] AW: Going back to Coot 0.8

Dear Herman, Am 09.09.2020 um 17:46 schrieb Schreuder, Herman /DE: The old real-space refinement was intuitive and easy to use and did exactly what the user expected, without having to consult the manual!😊 The result might not have been perfect, but was good enough for subsequent Refmac, Buste

Re: [ccp4bb] autoSHARP/SHELXD : Scatter plot program for OS X

ts (" .mtv " file format) from SHELXD/autoSHARP run in OS X Platform. Thank you, Raspudin -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: georg.zoc...@uni-tuebingen.de

Re: [ccp4bb] image file extensions

2.11 15:49, schrieb Andreas Förster: Dear all, I'm trying to create some space on our server and want to compress all x-ray data files. I'm wondering what extensions I should search for. mccd and img come to mind easily. What other extensions are commonly used? Thanks. Andreas -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: georg.zoc...@uni-tuebingen.de http://www.ifib.uni-tuebingen.de

Re: [ccp4bb] how to Collecting Data from Long Unit Cell Axes ?

. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: georg.zoc...@uni-tuebingen.de http://www.ifib.uni-tuebingen.de

Re: [ccp4bb] High B-factor for metal

nt by MailScanner, and is believed to be clean. -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: georg.zoc...@uni-tuebingen.de http://www.ifib.uni-tuebingen.de

Re: [ccp4bb] turn granular to crystal

nd also don't have sharp edges. See the attached pic. The current condition is PEG4000 and pH around 5. How can I improve this condition? Thanks a lot. -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)

Re: [ccp4bb] About SAD phasing

Hi Qing, just want to add that it also may depend on the redundancy of the data. While good MAD experiments were performed with low redundancy (best to use a strategy for data collection) a successful SAD experiment often depends on high redundancy. Best Regards, Georg Am 30.08.2010 20:58,

[ccp4bb] problem with anisotropic refinement using refmac

ed su of positional parameters = 0.0274 ML based su of thermal parameters= 1.5420 rmsBOND = 0.014 rmsANGLE = 1.569 Thanks in advance, georg zocher

Re: [ccp4bb] problem with anisotropic refinement using refmac

possiblities, but especially the tls refinement seems to be a good option to be worthy to try. Thanks for your helpful advices, georg Ethan Merritt schrieb: On Tuesday 30 January 2007 04:11, Georg Zocher wrote: I have a problem refining a structure against a 1.33 A resolution data [snip] To finish

Re: [ccp4bb] Self rotation function and figures

U Sam wrote: Hi, I am using a native data of 2 angstrom and C2 space group, final R-merge is 0.059. I processed data using HKL2000. I highly appreciate suggestion how can I generate self-rotation function, get figures and save that in required format. Thanks, Sam _

Re: [ccp4bb] How to create TLS file

=TXT_HMTG_prodredemoticons_052008 I recommend to use the TLSMD-server (J Painter & E A Merritt (2006) Acta Cryst. D62, 439-450) http://skuld.bmsc.washington.edu/~tlsmd/ best regards, georg -- Dr. Georg Zocher BESSY-MX group BESSY GmbH Albert-Einstein-Straße-15 D-12489 Berlin Fon : +49-(0)30-6392 4973 Fax : +49-(

Re: [ccp4bb] environ.def / default.def

.setup' if your shell is the (t)csh. but NOT BOTH. best regards, georg -- Dr. Georg Zocher BESSY-MX group BESSY GmbH Albert-Einstein-Straße-15 D-12489 Berlin Fon : +49-(0)30-6392 4973 Fax : +49-(0)30-6392 4975 email: [EMAIL PROTECTED] url : http://www.psf.bessy.de/ BESSY GmbH - Mit

[ccp4bb] .getTwoTheta() in DiffractionImage library

called 'catmar' showed the 'right' angle. Maybe I just made a very stupid error.... Thanks in advance, best regards Georg -- Dr. Georg Zocher BESSY-MX group BESSY GmbH Albert-Einstein-Straße-15 D-12489 Berlin Fon : +49-(0)30-6392 4973 Fax : +49-(0)30-6392 4975 email: [

Re: [ccp4bb] FW: [ccp4bb] .getTwoTheta() in DiffractionImage library

MAXIMUM_NUMBER_OF_PROCESSORS= "] = _get_ncpu() if debug == "on": print print " DEBUG OUTPUT == " print print "\t\t 2Theta readout: \t\t %.3f" %diff.getTwoTheta() print for key, va

[ccp4bb] Postdoctoral Fellow: Protein expression and X-ray structural analysis at ZMBP, Tuebingen

# Best Regards, Georg -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: georg.zoc...@uni-tuebingen.de http://www.ifib.uni-tuebingen.de

Re: [ccp4bb] Phasing at Low Resolution

(<=4A) of large complexes (>=150 kDA) - in terms of - derivatization compounds (is there something like the 'golden five' HA compounds for these cases), - data collection techniques and - phasing methods? Clemens -- Universität Tübingen Interfakultäres Institut für Bio

[ccp4bb] Post-doctoral Position in Structural Biology at IFIB

ingen +49-7071-2973043 thilo.ste...@uni-tuebingen.de <mailto:thilo.ste...@uni-tuebingen.de> -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: georg.zoc...@uni-tuebingen.de h

Re: [ccp4bb] SAD phasing at home source

rds, Georg james09 pruza schrieb: Dear All, I need some suggestions regarding the SAD phasing using home source. What the the most commonly used HA for SAD phasing for Cu anode. All suggestions are welcome. Thanks. James -- Universität Tübingen Interfakultäres Institut für Biochemie

[ccp4bb] PhD positions at the Interfaculty Institute of Biochemistry, University of Tuebingen

Dear CCP4BB-Users, On behalf of Prof. Thilo Stehle I would like to post a job offer for two PhD positions at the Interfaculty Institute of Biochemistry. Best Regards, Georg * * *# * *PhD positions in Structural Biology at the Interfaculty Institute of Biochemistry * We offer PhD posi

[ccp4bb] transport of fully functional virus crystals

.) How have you done the transport? 3.) Is there a dry shipper available which fulfills the requirements for packing instruction 650? Any comment is welcome. Thank you very much for your efforts. Cheers, Georg -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe

Re: [ccp4bb] transport of fully functional virus crystals

:* Georg Zocher *To:* CCP4BB@JISCMAIL.AC.UK *Sent:* Tue, July 17, 2012 12:45:22 PM *Subject:* [ccp4bb] transport of fully functional virus crystals Dear Community, I have to face the problem that I have to transport fully functional and infectious virus crystals (frozen) to a synchrotron. The

Re: [ccp4bb] transport of fully functional virus crystals

rmer (http://www.coleparmer.com/) and see what they recommend. They have been very helpful in the past. Kris Kris F. Tesh, Ph. D. Department of Biology and Biochemistry University of Houston ---- *From:* Georg Zocher *T

[ccp4bb] Two PhD positions at the Interfaculty Institute of Biochemistry - Prof. Thilo Stehle

information of two persons for references should be send as single pdf document (max. size 3 MB) to thilo.stehle[at]uni-tuebingen.de. -- Universität Tübingen Interfakultäres Institut für Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2975359 Mail: georg.zoc