Re: ccp4bb on new site

2007-01-19 Thread Douglas L. Theobald
On Jan 19, 2007, at 4:20 PM, Steve Lane wrote: Agreed. If at all possible, please resume use of the [ccp4bb] Subject: tag. I'll add another vote for the [ccp4bb] tag. -- Steve Lane System, Network and Security Administrator Doudna Lab Biomolecular Structure and Mechanism Group UC Berkele

[ccp4bb] Easy proteins to crystallize

2007-02-22 Thread Douglas L. Theobald
but nearly as nice). Douglas ^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^` Douglas L. Theobald Department of Biochemistry Brandeis University Waltham, MA 02454-9110 [EMAIL PROTECTED] ^\ /` /^. / /\ / / /`/ / . /` / / ' ' '

Re: [ccp4bb] Easy proteins to crystallize

2007-02-22 Thread Douglas L. Theobald
vironmentsthe purification protocol often involves a "boiling step" that denatures/degrades everything else in the prep...the resulting incredibly high purity and homogeneity may be a contributing factor... HTH, Iain Douglas L. Theobald wrote: Hi all, I'd like to pic

Re: [ccp4bb] Easy proteins to crystallize

2007-02-22 Thread Douglas L. Theobald
work with. On Thu, 22 Feb 2007, Douglas L. Theobald wrote: Hi all, I'd like to pick the collective brain of crystallographers on this list -- what are some of the most easily crystallizable proteins? I'm especially interested in those that over-express and diffract well, and in o

Re: [ccp4bb] Easy proteins to crystallize

2007-03-07 Thread Douglas L. Theobald
t be less well-known than, say, lysozyme (but nearly as nice). Douglas ^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^` Douglas L. Theobald Department of Biochemistry Brandeis University Waltham, MA 02454-9110 [EMAIL PROTECTED] ^\ /` /^. / /\ / / /`/ / . /` / / ' ' ' Check Out

Re: [ccp4bb] superpose

2007-03-12 Thread Douglas L. Theobald
an Juan Sanchez-Weatherby School of Biological Sciences University of East Anglia Norwich NR4 7TJ United Kingdom ^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^` Douglas L. Theobald Department of Biochemistry Brandeis University Waltham, MA 02454-9110 [EMAIL PROTECTED] GPG key ID: 38E9EB53 https://www.molevo.org/keys/38E9EB53.gpgkey ^\ /` /^. / /\ / / /`/ / . /` / / ' ' '

[ccp4bb] Easy proteins to crystallize -- summary

2007-03-21 Thread Douglas L. Theobald
Thanks all who answered, and sorry for the delay -- I was held up having a date with a lovely quasi-species of norovirus. I had asked the list for examples of proteins that are easily crystallizable, especially those that over-express and diffract well, not necessarily well-known. The cla

Re: [ccp4bb] rmsd calculation

2007-05-11 Thread Douglas L. Theobald
sum/ n}'\ lo=${lower} up=${upper}); echo "${name} rmsd = ${rmsd}"; done done ^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^` Douglas L. Theobald Department of Biochemistry Brandeis University Waltham, MA 02454-9110 [EMAIL PROTECTED]

Re: [ccp4bb] Kay Diederichs Email

2007-07-07 Thread Douglas L. Theobald
t this is a bug from Apple and not any fault of Kay's. Also, I probably wouldn't bother in general, but his emails are usually worth reading. James ^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^`^` Douglas L. Theobald Assistant Professor Department of Biochemistry Mailstop 009 415 Sou

Re: [ccp4bb] Kay Diederichs Email

2007-07-07 Thread Douglas L. Theobald
t about it soon enough. Ian On Jul 7, 2007, at 1:15 PM, Douglas L. Theobald wrote: Hey, I have the exact same problem. I haven't been able to read SMIME signed/encrypted emails now for some time (Mail just crashes). It is an intel apple bug of some sort. If you find the email in ~/L

Re: [ccp4bb] how to convert matrix to angle

2007-07-08 Thread Douglas L. Theobald
To add a bit to what others have already said -- The formula that Kay gave ((a11 + a22 + a33 - 1) / 2) throws out 2/3 of the rotational information. A 3D rotation cannot be described completely by a single angle: minimally three angles are actually required. There are many different ways,