[ccp4bb] UVEX UV Fluorescence

2017-04-05 Thread Cyprian Cukier
Dear All, We are about to buy a new imaging system for our laboratories and we consider UVEX UV Fluorescence Imaging from MD. Can anyone provide some comments about this equipment (eg. is it reliable, robust, no technical issues, low-maintenance etc.)? All comments will be appreciated. Than

Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Cyprian Cukier
I believe HRV3C might be more efficient if you need to cleave in the absence of reducing agents (e.g. extracellular proteins). Best regards, Cyprian Cyprian Cukier, PhD Selvita S.A. From: CCP4 bulletin board On Behalf Of Crissy L Tarver Sent: Wednesday, December 7, 2022 9:38 PM To: CCP4BB

Re: [ccp4bb] add ligand with AceDRG

2024-04-26 Thread Cyprian Cukier
to make sure that it is not related to your ccp4 installation. Best regards Cyprian Cyprian Cukier, Ph.D. Head of Structural Biology Selvita S.A. From: CCP4 bulletin board On Behalf Of Garib Murshudov Sent: Friday, April 26, 2024 1:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] add

[ccp4bb] microfluidizer

2025-04-05 Thread Cyprian Cukier
Dear Community, First of all, apologies for the slightly off-topic question, which is related to protein purification rather than crystallography. I am seeking your advice on preparing the cell pellets for the lysis process. We have an LM20 microfluidizer and we experience quite frequent cloggi

Re: [ccp4bb] microfluidizer

2025-04-01 Thread Cyprian Cukier
ry to unclog the cell mounted in a reverse way. Best. Michel. Le 25 mars 2025 à 07:09, Cyprian Cukier <mailto:cyprian.cuk...@selvita.com> a écrit :  Dear Community, First of all, apologies for the slightly off-topic question, which is related to protein purification rather than cryst