I believe HRV3C might be more efficient if you need to cleave in the absence of reducing agents (e.g. extracellular proteins).
Best regards, Cyprian Cyprian Cukier, PhD Selvita S.A. From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Crissy L Tarver Sent: Wednesday, December 7, 2022 9:38 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] TEV vs HRV3C We use the HRV3C protease site frequently in our lab. It provides better on-bead/column cleavage than TEV. We have a plasmid as we make 3C protease in our lab and keep stocks in our -80C. I can send you our protocol if you wish. Crissy Crissy L Tarver, PhD Department of Structural Biology Stanford University School of Medicine Confidentiality notice: This communication and any attachments may contain confidential or privileged information for the use by the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or the attachments is strictly prohibited. If you have received this communication in error, please contact me and destroy all copies of the communication and attachments. Thank you. ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of Gloria Borgstahl <gborgst...@gmail.com<mailto:gborgst...@gmail.com>> Sent: Wednesday, December 7, 2022 12:26 PM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: [ccp4bb] TEV vs HRV3C Hello my fellow structural biologists, I am contemplating why some choose the HRV3C protease site over TEV for their fusion proteins. Does anyone know? Can HRV3C be made easily in homelab? Does anyone have a plasmid? Thank you, G ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
