Dear Community, First of all, apologies for the slightly off-topic question, which is related to protein purification rather than crystallography.
I am seeking your advice on preparing the cell pellets for the lysis process. We have an LM20 microfluidizer and we experience quite frequent clogging of the system that is tedious to remove. This happens particularly with the E. coli pellets, but from time to time the eukaryotic cells are also problematic. Does anyone have well-established protocols on how to handle the cell pellets before applying them to the lysis process in the microfluidizer? I should add that of course we follow the manufacturer's recommendations to have a homogenous suspension, which is not too dense. I would also appreciate any advice on how you clean your systems (regularly or in case of clogging). Best regards Cyprian ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
