Re: [ccp4bb] Cadmium sites and co-ordinations in structure

While it's not a review on protein binding sites, the following review might be helpful in understanding its chemistry: Holloway & Melnik (1995) Main Group Met. Chem. 451-585. Also, David Giedroc has written a couple of useful reviews that include discussions of cadmium binding proteins. Cadmi

Re: [ccp4bb] Bio Rad "Econo" system

I've been using the Econo system for about 20 years for prep scale purifications. I regularly do gradients using an external gradient maker (something like with Hoefer sells - http://www.hoeferinc.com/product.asp?ProdID=171) that feeds into the pump. It's worked reliably for me and allows the

Re: [ccp4bb] off topic: problematic protein

the presence of 300 mM NaCl). Worked great, and I saw stuff coming off the column in both washes. The reference is: Bayburt et al. (2002) Nano Letters, vol. 2, pp 853-856. http://pubs.acs.org/doi/abs/10.1021/nl025623k Good luck, Arthur Arthur Glasfeld Department of Chemistry Reed College 3203 SE

Re: [ccp4bb] Unexplained density near cobalt

s we could see both positions occupied within the same crystal (it looked a lot like what you are seeing under those circumstances). Peaks in the anomalous difference Fourier maps were the clearest evidence that it was manganese in each of the positions. Good luck, Arthur Arthur Glas

[ccp4bb] crystals as carry-on items

any recent experience with the TSA that might make this an easier task? Is it even possible any more? Thanks in advance for any advice that can be provided. Arthur Glasfeld Department of Chemistry Reed College 3203 SE Woodstock Blvd. Portland, OR 97202 USA

Re: [ccp4bb] Protein binding to Zn and Ca

Thermolysin binds a catalytic zinc and several Ca ions for structural stability. See Holland et al. (1995) Protein Sci, 4, 1955. Hope that helps, Arthur Glasfeld Department of Chemistry Reed College 3203 SE Woodstock Blvd. Portland, OR 97202 USA On Jun 12, 2008, at 8:15 PM, Neeraj Kapoor

Re: [ccp4bb] Manganese buffers for protein crystallization

s seems to promote its formation. That said, I've had xtals that diffract very well, with bound Mn, come out of drops containing the precipitate. Good luck, Arthur Arthur Glasfeld Department of Chemistry Reed College 3203 SE Woodstock Blvd. Portland, OR 97202 USA On Apr 6, 2009, at 11

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

ified that the crystals survived the treatment in the solutions by eye as well). Only tried it that once, so I have no idea how reliable it is. Good luck, Arthur Glasfeld Department of Chemistry Reed College 3203 SE Woodstock Blvd. Portland, OR 97202 USA On Sep 22, 2009, at 1:24 AM, Claudia S

[ccp4bb] Off-topic, visualization

ction.jpg Does anyone know of any software that can produce similar images? Thanks, Arthur Glasfeld Reed College Portland, OR

Re: [ccp4bb] Unexplainable Density

You might be interested in some work done on NikA, which binds a nickel complex with a small organic ligand. They tentatively identified the ligand as butane-1,2,4-tricarboxylate. http://www.rcsb.org/pdb/explore.do?structureId=3e3k Good luck, Arthur On Aug 8, 2012, at 4:56 AM, Mario Sniady w