I've been using the Econo system for about 20 years for prep scale purifications. I regularly do gradients using an external gradient maker (something like with Hoefer sells - http://www.hoeferinc.com/product.asp?ProdID=171) that feeds into the pump. It's worked reliably for me and allows the use of cheap columns to boot.
Good luck, Arthur On Feb 3, 2012, at 4:08 PM, narayan viswam wrote: > Dear CCP4BB, > > Sorry for this off-topic question. However it would immensely help our not > so greatly well-funded lab if people could share their experiences regarding > Bio-Rad econo pump system. > > We are planning to buy the Bio Rad "Econo" system or the Bio Rad "Bio Logic" > system. They are basically the same thing, but the Bio Logic is a bit > fancier because it can be controlled by a windows pc. > > We will be using this for preparative protein purification on the order of 1 > to 10 mg of purified protein. We require a method of for gradient formation > for ion-exchange and hydrophobic interaction chromatography. > > Opinions on this system from other academic or commmericial sector scientists > who have used this apparatus would be greatly appreciated. > > > Thanks a lot. > > Narayan
